Generator

Part:BBa_I766381

Designed by: Michael Chen   Group: iGEM07_UCSF   (2007-10-25)

OspF-RR under medium constituitive promotor

OspF-RR under medium constituitive promotor

Usage and Biology

OspF is a bacterial phosphothreonine lyase, apparently specific for MAPKs. It catalyzes the removal of the phosphorylated threonine side chain present in the MAPK activation loop, therefore inactivating the kinase in an irreversible manner. Here, OspF is N-terminally fused to mCherry, and C-terminally to the synthetic leucine zipper RR; being expressed under control of a Cyc1 promoter (constitutive medium level expression).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1348
    Illegal NotI site found at 1907
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 820
    Illegal BglII site found at 904
    Illegal BamHI site found at 1890
    Illegal XhoI site found at 988
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1348
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None